I have a 3 year old Leonberger male, intact and never been bred. He had
semen collected at a dog show in October 2018 which showed high numbers of
immotile sperm. He was then referred to me. The dog had great libido and gave a
very white second fraction, with clear first and third fractions. Sperm count
was over 2 billion, but 99% immotile. Morphology showed about 4% normal, 40%
distal droplets, 20% proximal droplets, 18% bent mid pieces, 13% abnormal head
shapes, and 3% coiled tails. Viability measured by PI on NucleoCounter was 19%.
Aerobic culture of semen (manual collection) yielded no growth.
Mycoplasma/Ureaplasma culture at Cornell yielded no growth. SCSA testing at
Texas A&M was within normal ranges. Male is brucellosis negative on RSAT.
He is not on a grain-free diet.
We gave the dog 4 months of rest and rechecked his semen. No appreciable
I have now collected him 3 days in a row (and slated to continue
tomorrow), with only a slight change in motility (0.4% to 2.5% motility) and no
appreciable change in morphology. Overall sperm numbers have remained high
(dropping from 2 billion to 1.2 billion).
The male reportedly had retained testes until he was 13 weeks old. The
testes now are uniformly shaped, slightly soft, appropriate size, with no
lesions on ultrasound.
Looking for advice on a
case(s) I was presented with this week.
First Case: On Tuesday I
was referred by a DVM (also original owner of bull) a yearling bull that passed
a satisfactory BSE in January (for sale) and then was recollected by the buyers
DVM 2 weeks ago and the DVM noticed “blood in the ejaculate and told the
buyer to take it back to the seller (DVM) and of course without any information
of the BSE. The seller sent it my direction for a second evaluation. Everything
physically with the bull was adequate. The ejaculate was brown in color (see
attached images). Testicles and all accessory sex glands were ultrasounded with
normal findings. Any idea what this would be caused by? I have seen frank blood
in ejaculates but nothing like this. I assume this is just metabolized blood
(hemoglobin) giving me this color since on the motility I was not seeing any
small cells resembling RBCs. I also assume that this is coming from an
accessory sex gland (even though palpation and ultrasound did not reveal any
changes)and not the urinary tract since sperm cells were 80% progressively
motile (see video attached) with 84% normal morphology, and not dead from urine
contaminants. Also, is anyone else seeing ejaculates like this? I ask because
the following day, I was doing 8 bulls from a different farm unrelated to the
previous bull and one of the bulls gave me identical sample with similar exam
findings. Interestingly enough this second bull would give me what looked like
a normal ejaculate (white) and then would discharge brown colored fluid. We
repeated both bulls with same results.
Any suggestions on how to
figure out where the brown ejaculate comes from? Which tests? Chemistry profile
(and is there a good reference of what is normal)?
Nutritionally, the first
bull is getting fed a TMR with silage/hay and corn, other is on grass for the
past 3 weeks. Is there a potential of a toxin or nutritional component?
My thoughts: coming from
accessory sex gland (seminal vesicles) since we see yellow/orange tinge
coloring from yearlings and others that concentrate riboflavin-niacin complex,
early onset vesiculitis (even though no WBCs noted on microscopic evaluation)?.
I put both bulls on
meloxicam (for possible unknown inflammation) for now and told owners I would
investigate my lack of knowledge.
Any insight or help would
be much appreciated. Been racking my brain to try and figure this out since I
saw 2 in less than 24 hours and have no good explanation.