Re Semen Storage Regulations (from ACT Listserv)

Does anyone know of any regulations, or protection, regarding semen that is stored and the owner is unknown, or unable/unwilling to retrieve or pay storage fees?

Reply 1. You would be surprised how often owners forget they have semen stored. Or folks pass away leaving no record.

Reply 2. Not aware of any regulations per se, although when it comes to export work there are many rules concerning health, testing, biosecurity, quarantine, facility and subsequent storage protocols. Otherwise storage of other people’s property comes under “generally practiced” guidelines such as a signed storage agreement and disclosure of insurance (or not). Insurance can be obtained covering loss – if not, clients should be informed (certified mail?) that there is no protection against damage, accident or loss. A business policy should include such coverage, however.

Reply 3. “We have a 5 page storage agreement which specifies that, if an entire billing period is unpaid (following due notice) then we will not be responsible for further storage and may, at our option, elect to sell to recover accrued storage costs”

Reply 4. “I hope you have more luck with certified mail than we did. Clients can decline to sign for it, then claim that they did not receive it. Thus there is no proof unless you have the envelope. Maybe we could camouflage the envelope to say “You have won an all-expense paid trip to Hawaii etc”?

Question re freezing ram semen

When we collect ram semen to freeze (we use Triladyl), we usually find that the first collection each day does not freeze as well as the later collection, even though semen quality is apparently the same. So..we often discard the first collection on principle, and freeze the subsequent collections.

Not sure what cause this this variability, perhaps differences in seminal plasma?

Is this a common, or reasonable policy. Or are we throwing out good semen?

Blastomycosis and dog semen

Blastomycosis and Semen

I got a call from a gentleman who owns a male intact German Shorthair that was diagnosed with blastomycosis. They collected and froze semen before starting treatment and the bitch to whom the dog is to be bred is now is season. The owner wants to know if blasto is transmissible through that semen. I know dogs with blasto shed tons of organisms into urine but cannot find any info about shedding into semen, likelihood of blasto surviving the freeze/thaw process, or likelihood of infection of the female via introduction of organisms in her uterus. Any suggestions are much appreciated!

Another frustrating dog problem

Now I have a weird dog question  

This is from a 3 yr old dog, no history of illness. He has been out on the show circuit this year and it is hot (85+F consistently for the past 2+ months), though he lives in an air-conditioned kennel or in the house. 

This was a routine semen check prior to offering him at stud. Motility & concentration were good. However, >95% of his heads had this ‘balloon’ shape that tapers sharply to the midpiece. He had a potpourri of other abnormalities, TCT, swollen/irregular midpieces, but nothing out of the ordinary. I have seen these heads occasionally in other dogs and considered them abnormal, but never in the percentages that this dog has them.

Could this be normal for him? I gave the owner options of rechecking in 2-3 months (accounting for sperm cycle & weather) or finding a bitch to do a test breeding. 
Has anyone seen these type of heads so prevalent in dogs that were fertile?

Canine semen question

Dear Colleagues,

I have a case that is frustrating me. 

I have a 3 year old Leonberger male, intact and never been bred. He had semen collected at a dog show in October 2018 which showed high numbers of immotile sperm. He was then referred to me. The dog had great libido and gave a very white second fraction, with clear first and third fractions. Sperm count was over 2 billion, but 99% immotile. Morphology showed about 4% normal, 40% distal droplets, 20% proximal droplets, 18% bent mid pieces, 13% abnormal head shapes, and 3% coiled tails. Viability measured by PI on NucleoCounter was 19%. Aerobic culture of semen (manual collection) yielded no growth. Mycoplasma/Ureaplasma culture at Cornell yielded no growth. SCSA testing at Texas A&M was within normal ranges. Male is brucellosis negative on RSAT. He is not on a grain-free diet. 

We gave the dog 4 months of rest and rechecked his semen. No appreciable change 

I have now collected him 3 days in a row (and slated to continue tomorrow), with only a slight change in motility (0.4% to 2.5% motility) and no appreciable change in morphology. Overall sperm numbers have remained high (dropping from 2 billion to 1.2 billion).

The male reportedly had retained testes until he was 13 weeks old. The testes now are uniformly shaped, slightly soft, appropriate size, with no lesions on ultrasound. 

Any thoughts?

Thank you!

Bull brown semen

Brown semen from a bull

Looking for advice on a case(s) I was presented with this week.

First Case: On Tuesday I was referred by a DVM (also original owner of bull) a yearling bull that passed a satisfactory BSE in January (for sale) and then was recollected by the buyers DVM 2 weeks ago and the DVM noticed “blood in the ejaculate and told the buyer to take it back to the seller (DVM) and of course without any information of the BSE. The seller sent it my direction for a second evaluation. Everything physically with the bull was adequate. The ejaculate was brown in color (see attached images). Testicles and all accessory sex glands were ultrasounded with normal findings. Any idea what this would be caused by? I have seen frank blood in ejaculates but nothing like this. I assume this is just metabolized blood (hemoglobin) giving me this color since on the motility I was not seeing any small cells resembling RBCs. I also assume that this is coming from an accessory sex gland (even though palpation and ultrasound did not reveal any changes)and not the urinary tract since sperm cells were 80% progressively motile (see video attached) with 84% normal morphology, and not dead from urine contaminants. Also, is anyone else seeing ejaculates like this? I ask because the following day, I was doing 8 bulls from a different farm unrelated to the previous bull and one of the bulls gave me identical sample with similar exam findings. Interestingly enough this second bull would give me what looked like a normal ejaculate (white) and then would discharge brown colored fluid. We repeated both bulls with same results.

Any suggestions on how to figure out where the brown ejaculate comes from? Which tests? Chemistry profile (and is there a good reference of what is normal)?

Nutritionally, the first bull is getting fed a TMR with silage/hay and corn, other is on grass for the past 3 weeks. Is there a potential of a toxin or nutritional component?

My thoughts: coming from accessory sex gland (seminal vesicles) since we see yellow/orange tinge coloring from yearlings and others that concentrate riboflavin-niacin complex, early onset vesiculitis (even though no WBCs noted on microscopic evaluation)?.

I put both bulls on meloxicam (for possible unknown inflammation) for now and told owners I would investigate my lack of knowledge. 

Any insight or help would be much appreciated. Been racking my brain to try and figure this out since I saw 2 in less than 24 hours and have no good explanation.

Thanks in advance,

Interesting frozen semen problem

This question came from an A.I. Centre in India.

3 Gir bulls, Semen Processing Centre in India. Collected together, processed together (Andromed), same LN container. Same canister?

Problem in post thawing motility in one of these bulls, only.

Post-thaw motility (PTM) of sperm similar in all bulls immediately following freezing and up to 1 week later. Then, gradually decreases, viz

PTM

 – after freezing, 50 %
– > one month, 35-40 %

– > 2 months, 20-25 %
– > 3 months, 10-15 %

– > 4-5 months, 0 %

PTM does not change over this time in the other 2 Gir bulls.

Suggestions, solutions?